The EU must "urgently clarify its intentions" after it brought in export controls on Covid vaccines to Northern Ireland, Boris Johnson has said.
The PM said the bloc must explain what it plans to do to ensure it honours its commitments to Northern Ireland.
It comes amid an escalating row between the EU and vaccine producer AstraZeneca over delivery shortfalls.
The EU said the move was "justified" to avert problems caused by a lack of vaccine supply2.
The BBC's Europe editor Katya Adler said EU sources have told her that the European Commission was going to backtrack on its decision and would not suspend part of the Northern Ireland protocol.
The source told our correspondent that "suggesting to do that was misjudgement and an error".
On Friday, the bloc invoked Article 16 of the Northern Ireland Protocol which allows parts of the deal to be unilaterally overridden.
In the new regulation, the European Commission stated: "This is justified as a safeguard measure pursuant to Article 16 of that Protocol in order to avert serious societal difficulties due to a lack of supply threatening to disturb the orderly implementation of the vaccination campaigns in the Member States."
The EU's move should not directly disadvantage Northern Ireland, which gets its vaccine supplies through the UK procurement system.
The EU's actions have been criticised by a string of politicians, with Northern Ireland's First Minister Arlene Forster describing it as "an incredible act of hostility" that places a "hard border" between Northern Ireland and the Republic of Ireland.
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